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Rapid one-step biotinylation of biological and non-biological surfaces

Nature Scientific Reports Open Access – https://doi.org/10.1038/s41598-018-21186-3

Summary: We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells.

Stephen Henry, Eleanor Williams, Katie Barr, Elena Korchagina, Alexandr Tuzikov, Natalia Ilyushin, Sidahmed A. Abayzeed, Kevin F. Webb & Nicolai Bovin, Scientific Reports (2018), 8:2845

Abstract

We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells.